Transcription Factor

Transcription factor is a protein that binds to the specific DNA sequences, thereby controlling the rate of transcription of genetic information from DNA to messenger RNA. Transcription factors perform this function alone or with other proteins in a complex, by promoting (as an activator), or blocking (as a repressor) the recruitment of RNA polymerase (the enzyme that performs the transcription of genetic information from DNA to RNA) to specific genes.

A defining feature of transcription factors is that they contain one or more DNA-binding domains, which attach to specific sequences of DNA adjacent to the genes that they regulate. Here, we identify 52 families of transcription factors in the kiwifruit genome.

AP2 ARF RAV BEL1 bHLH BTF3 bZIP C2H2 C3H CAMTA
CBF Dof E2F/DP EIL ERF FAMA FAR1 GAMYB GATA GLK2
GRAS GRF GTE HB-PHD HD-ZIP HLTF HSF KAN KNOX LEC1
MADS MYB Myelin NAC NF-X1 NF-YA NF-YB NF-YC PALM1 PCL
POU PTF SCL TCP TFIIB TFIIIA Trihelix TT2 VOZ WRKY
YABBY ZF-HD                

Auxin response factors (ARF) are transcription factors that regulate the expression of auxin response genes. ARFs bind with specificity to TGTCTC auxin response elements (AuxRE) in promoters of these genes and function in combination with Aux/IAA (auxin/indole acetic acid) repressors, which dimerize with ARF activators in an auxin-regulated manner.

Most ARFs consist of an amino-terminal DNA-binding domain (DBD), a middle region that functions as an activation domain (AD) or repression domain (RD), and a carboxy-terminal dimerization domain (CTD). The ARF DBD is classified as a plant-specific B3-type, but requires additional amino-terminal and carboxy-terminal amino acids for efficient in vitro binding to TGTCTC AuxREs.

The ARF ADs and RDs are located just carboxy-terminal to the DBDs and contain biased amino acid sequences. ARF ADs are enriched in glutamine along with serine and leucine residues, while ARF RDs are enriched in serine, proline, leucine and glycine residues.

Protein name Gene name Description Pathway Interaction
Protein name Gene name Description Pathway Interaction
Ach00g043361.2Ach00g043361Uncharacterized proteinK14486:K14486, ARF0
Ach00g312371.2Ach00g312371Uncharacterized protein0
Ach00g344691.2Ach00g344691Uncharacterized proteinK14486:K14486, ARF0
Ach01g285231.1Ach01g285231Uncharacterized protein0
Ach07g346141.2Ach07g346141Uncharacterized protein1
Ach11g049931.2Ach11g049931Uncharacterized protein1
Ach16g216581.2Ach16g216581Uncharacterized protein1
Ach25g177821.2Ach25g177821Uncharacterized protein1